A near-infrared magnetic aptasensor for Ochratoxin A based on near-infrared upconversion nanoparticles and magnetic nanoparticles

A multiplexed, sensitive and specific detection method is highly desirable for the simultaneous detection of several pathogenic bacteria and bio-toxins. In our previous work, multicolor upconversion nano-particles (UCNPs) via doping with various rare-earth ions to obtain well-separated emission peaks by means of a solvothermal method were synthesized and were successfully applied as luminescence labels in the detection of three pathogenic bacteria. One of the basic achievements of our group has been to establish that the key to increasing the number of simultaneous detection components is the preparation of more UCNPs, the emission peaks of which can be distinguished from each other. According to this vision, NaYF4:Yb-0.2, Tm-0.02 UCNPs were obtained via a thermal-decomposition protocol, which has a main near-infrared (NIR) UC emission at 804 nm under 980 nm excitation. The emission peak at 804 nm was well-separated from the emission peaks of UCNPs we have reported at 477 nm, 542 nm, and 660 nm. It means both the excitation and the emission of NaYE4:Yh(0.2), Tm-0.02 UCNPs are located in the NIR spectral range (NIR-to-NIR UC emission), the so-called biological window. This result establishes the basis of achieving simultaneous detection of four components. To confirm the analytical performance of this NaYF4:Yb-0.2, Tm-0.02 UCNPs, a novel near-infrared magnetic aptasensor for the detection of Ochratoxin A (OTA) was developed using the OTA aptamer-conjugated near-infrared upconversion nanoparticles (apt-UCNPs) and the complementary oligonucleotide-modified magnetic nanoparticles (cDNA-MNPs). The apt-UCNPs and cDNA-MNPs were hybridized to form a poly-network structure of MNP-UCNP nano-composites. When the target OTA was introduced, the aptamer combined with the priority target and the cDNA-MNPs were replaced. The proposed method achieved a linear range between 0.01 and 100 ng mL(-1), with a detection limit as low as 0.005 ng mL(-1). Then, we successfully applied this method to measure Ochratoxin A (OTA) in beer samples and the results demonstrated that the method possessed a high sensitivity and good selectivity for the determination of OTA and thus is applicable to the determination of OTA in beer samples. This satisfying result shows that the NaYF4:Yb-0.2, Tm-0.02 UCNPs we synthesized has a great prospect in multiplexed simultaneous detection. (C) 2016 Elsevier B.V. All rights reserved.