Journal
TALANTA
Volume 159, Issue -, Pages 1-6Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.05.075
Keywords
MOF; Phosphopeptide enrichment; Lanthanide; Mass spectrometry
Categories
Funding
- National Basic Research Priorities Program [20120910602, 20130911201]
- National Natural Science Foundation of China [21425518, 21075022, 21275033, 21105016]
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 20100071120053]
Ask authors/readers for more resources
Highly efficient enrichment of phosphopeptides from complex biological samples is crucial prior to mass spectrometry analysis due to the low abundance and ion suppression effects. In this study, a facile route was designed for preparation of a magnetic erbium(Er)-based metal-organic framework (denoted as Fe3O4@PDA@Er(btc)), which was synthesized with 1,3,5-benzenetricarboxylic acid(H(3)btc) as ligand and grafted on the polydopamine (PDA) - coated Fe3O4. The as-prepared material exhibited ultra-high sensitivity (detection limit of 20 amol/mu L) and selectivity at a low mass ratio of beta-Casein/BSA (1:500). Moreover, it was also investigated for enrichment of phosphopeptides from human serum, which provided a promising technique for highly efficient enrichment of low-abundance phosphorylated peptides in the practical application. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available