Journal
TALANTA
Volume 150, Issue -, Pages 485-492Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.12.051
Keywords
Quantitative H-1-NMR; P-31-NMR; ATP; ADP; AMP; Serum
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Funding
- Analytical Platform Construction Project of Key Scientific Production of Sichuan Province, China [12CGPT0022]
- Analytical Platform Construction Project of Scientific Production of Sichuan Province, China [13CGPT0049]
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ATP, ADP and AMP are energy substances with vital biological significance. Based on the structural differences, a simple, rapid and comprehensive method has been established by H-1 and P-31 Nuclear Magnetic Resonance (H-1-NMR and P-31-NMR) spectroscopies. Sodium 3-(trimethylsilyl) propionate-2,2,3,3-d(4) (TMSP) and anhydrous disodium hydrogen phosphate (Na2HPO4) were selected as internal standards for H-1-NMR and P-31-NMR, respectively. Those three compounds and corresponding internal standards can be easily distinguished both by H-1-NMR and P-31-NMR. In addition, they all have perfect linearity in a certain range: 0.1-100 mM for H-1-NMR and 1-75 mM for P-31-NMR. To validate the precision of this method, mixed samples of different concentrations were measured. Recovery experiments were conducted in serum (91-113% by H-1-NMR and 89-113% by P-31-NMR). (C) 2015 Elsevier B.V. All rights reserved.
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