Journal
TALANTA
Volume 161, Issue -, Pages 482-488Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2016.08.081
Keywords
BSA-stabilized gold nanoclusters; Amino-functionalized graphene oxide; Energy transfer; Heparin; Intracellular imaging
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Funding
- National Natural Science Foundation of China (NSFC) [21035005]
- Fundamental Research Funds for the Central Universities [XDJK2016C177]
- Doctoral Scientific Research Foundation [SVVU116006]
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An ultra-sensitive and selective turn off-on fluorescence detection of heparin based on the energy transfer in the BSA-stabilized gold nanoclusters/amino-functionalized graphene oxide (BSA-AuNCs/NH2-GO) hybrids was successfully realized. The BSA-AuNCs containing amounts of carboxyl groups could be absorbed on the surface of NH2-GO through the electrostatic interaction, which resulted in the fluorescence quenching of BSA-AuNCs with high efficiency. However, heparin, possessing high density of negative charge, could compete with BSA-AuNCs to bind NH2-GO and block the energy transfer from BSA-AuNCs to NH2-GO. The fluorescence recovery of BSA-AuNCs was closely related to the amount of heparin and there was a good linear relationship between fluorescence recovery of BSA-AuNCs and heparin over the range of 100 ng/mL to 30 mu g/mL with a detection limit of 40 ng/mL. What's more, the fluorescence assay was successfully applied for heparin sensing in human serums and intracellular imaging. (C) 2016 Elsevier B.V. All rights reserved.
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