Journal
TALANTA
Volume 161, Issue -, Pages 271-277Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.08.060
Keywords
Electrochemiluminescence; Resonance energy transfer; Peptide; Prostate specific antigen (PSA); PAMAM dendrimer
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Funding
- National Natural Science Foundation [21305068]
- Natural Science Foundation of Jiangsu Province [BK20130666]
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Here, we propose a simple and novel signal-on electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) for detection of prostate specific antigen (PSA). The system is composed of Multi-walled carbon nanotubes (MWCNT), polyamidoamine (PAMAM) dendrimer and Au nano particles (NPs) film on glassy carbon electrode (GCE) to improve the electron transfer, provide abundant amine group for the immobilization of biomolecules, and amplify the ECL signal. After that, Au nanorods (Au NRs) labeled peptide is modified on electrode surface to serve as ECL-RET acceptor due to the excellent overlap between the ECL emission spectrum of Ru(bpy)(3)(2+) and the absorption spectrum of Au NRs, leading to the significant decrement of ECL signal. Upon the sensing cleavage of peptide with PSA, both Au NRs and peptide are released from electrode surface, resulting in the high recovery efficiency of ECL signal. The proposed approach exhibits a wide linear range from 0.1 pg/mL to 10 ng/mL with a detection limit of 0.03 pg/mL. Results revealed that the recoveries were in a range from 95% to 108%, indicating good accuracy of the proposed method for PSA detection. In addition, the proposed biosensor exhibited well specificity for the detection of PSA. (C) 2016 Elsevier B.V. All rights reserved.
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