Journal
TALANTA
Volume 161, Issue -, Pages 48-53Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.08.030
Keywords
Leishmaniasis; DNA biosensor; Genosensor; Gold nanoleaves
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Funding
- Research Council of Shiraz University of Medical Sciences
- Iran National Science Foundation (INSF)
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Detection of leishmaniasis is important in clinical diagnoses. In the present study, identification of Leishmania parasites was performed by a label-free, PCR-free and signal-on ultrasensitive electrochemical DNA biosensor. Gold nanoleaves were firstly electrodeposited by an electrodeposition method using spermidine as a shape directing agent. The biosensor was fabricated by immobilization of a Leishmania major specific DNA probe onto gold nanoleaves, and methylene blue was employed as a marker. Hybridization of the complementary single stranded DNA sequence with the biosensor under the selected conditions was then investigated. The biosensor could detect a synthetic DNA target in a range of 1.0 x 10(-10) to 1.0 x 10(-19) mol L-1 with a limit of detection of 1.8 x 10(-20) mol L--1,L- and genomic DNA in a range of 0.5-20 ng mu L-1 with a limit of detection of 0.07 ng mu L-1. The biosensor could distinguish Leishmania major from a non-complementary-sequence oligonucleotide and the tropica species with a high selectivity. The biosensor was applicable to detect Leishmania major in patient samples. (C) 2016 Elsevier B.V. All rights reserved.
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