Journal
TALANTA
Volume 147, Issue -, Pages 468-472Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.10.026
Keywords
Fluorescent probe; 7-Nitrobenzoxadiazole (NBD); Redox cycle; ClO-; Bioimaging
Categories
Funding
- National Natural Science Foundation of China [20972170, 21275150]
- Funds for Distinguished Young Scientists of Gansu [1210RJDA013]
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Based on the reversible redox reaction of 4-amino-2,2,6,6-tetramethyl-piperidin-1-ol with oxidative ClO- and reductive ascorbic acid (AA), a reversible fluorescent probe, incorporating 4-amino-2,2,6,6-tetramethyl-piperidin-1-ol group into a 7-nitrobenzoxadiazole (NBD) fluorophore, was synthesized and studied for the cyclic detection of ClO-/AA in aqueous solution. The NBD-based probe displayed sensitive and selective On-Off-On fluorescent responses to ClO-/AA and was successfully applied to monitor the ClO-/AA redox cycle in living HeLa cells under physiological conditions. (C) 2015 Elsevier B.V. All rights reserved.
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