Journal
TALANTA
Volume 146, Issue -, Pages 689-693Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.06.041
Keywords
Mesoporous titania; Immobilized enzyme; Hydrogen peroxide; Composite electrode; Amperometry
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Funding
- Fund for Scientific Research - Flanders (FWO) [G.0687.13]
- GOA-BOF [UA 2013-2016, 28312]
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An adhesive conducting electrode material containing of graphite, biocompatible ion exchange polymer nafion (R) and commercial mesoporous TiO2 impregnated with horseradish peroxidase (HRP) is prepared and characterized by amperometric, UV-vis and N-2 sorption methods. The factors influencing the performance of the resulting biosensor are studied in detail. The optimal electrode material consists of 45% graphite, 50% impregnated HRP-TiO2 and 5% nafion (R). The optimum conditions for H2O2 reduction are an applied potential of -0.3 V and 0.1 mM hydroquinone. Sensitivity and limit of detection in the optimum conditions are 1 A M-1 cm(-2) and 1 mu M correspondingly. The N-2 sorption results show that the pore volume of TiO2 decreases sharply upon adsorption of HRP. The preparation process of the proposed enzyme electrode is straightforward and potentially can be used for preparation of carbon paste electrodes for bioelectrochemical detections. (C) 2015 Elsevier B.V. All rights reserved.
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