Journal
TALANTA
Volume 151, Issue -, Pages 62-67Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.01.025
Keywords
Hg2+; Hybridization chain reactions; Magnetic Fe3O4 nanoparticles; Fluorescence
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Funding
- National Natural Science Foundation of China [21507034, 20977026]
- Hunan Provincial Natural Science Foundation of China [14112045]
- Scientific Research Fund of Hunan Provincial Education Department [13k017]
- Central Public-interest Scientific Institution Basal Research Fund [2006001001009]
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In this manuscript, the authors molecularly engineered a hybridization chain reactions (HCRs) based probe on magnetic Fe3O4 nanoparticles for the sensitive detection of Hg2+. The sensing system comprised three probes: capture probe H1, report probe H2, and report probe H3. The capture probe was modified on the surface of magnetic Fe3O4 nanoparticles. The report probes were labeled with fluorescein isothiocyanate (Flit). Without Hg2+, the report probes were stable as molecular beacons in solution. In the presence of Hg2+, the T-rich capture probes and report probes will hybridize into double-helical DNA domains with the aid of T-Hg2+-T coordination chemistry. Trigged by this reaction, more molecular beacons open and form a super tandem structure. Herein, the fluorescence signal was magnified by capturing more report probes. Separating the target and captured report probes from reaction solution was benefit to decrease the background signal and interference from other metal ions. The detection limit of this method was about 0.36 nM, which is much lower than the regulations of World Health Organization and U.S. Environmental Protection Agency on Hg2+ in drink water. This proposed sensing strategy also showed favorable selectivity over other common metal ions. In addition, it has good practicability in real water samples. (C) 2016 Elsevier B.V. All rights reserved.
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