Journal
CLINICA CHIMICA ACTA
Volume 438, Issue -, Pages 67-69Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2014.08.004
Keywords
Immunoglobulin IgM; Oligoclonal bands; Multiple sclerosis; Biomarker
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Funding
- Instituto de Salud Carlos III (Spain)-Fondo Europeo de Desarrollo Regional (Feder) [PI12/00239]
- Ministerio de Economia y Competitividad (Spain) [SAF2012-34670]
- Instituto de Salud Carlos III, Spanish Ministry of Economy and Competitiveness [PT13/0010/0041]
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Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. 2014 Elsevier B.V. All rights reserved.
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