Journal
STRUCTURE
Volume 24, Issue 6, Pages 965-976Publisher
CELL PRESS
DOI: 10.1016/j.str.2016.03.026
Keywords
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Funding
- Intramural Research Program of the NIH, National Institute of Diabetes and Digestive and Kidney Diseases
- National Cancer Institute
- National Center for Biotechnology Information
- National Science Foundation [MCB-1452464]
- NSF [OCI-1053575]
- US Department of Energy, Office of Science, Office of Basic Energy Sciences
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1452464] Funding Source: National Science Foundation
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Incorporation of lipopolysaccharide (LPS) into the outer membrane of Gram-negative bacteria is essential for viability, and is accomplished by a two-protein complex called LptDE. We solved crystal structures of the core LptDE complexes from Yersinia pestis, Klebsiella pneumoniae, Pseudomonas aeruginosa, and a full-length structure of the K. pneumoniae LptDE complex. Our structures adopt the same plug and 26-strand beta-barrel architecture found recently for the Shigella flexneri and Salmonella typhimurium LptDE structures, illustrating a conserved fold across the family. A comparison of the only two full-length structures, SfLptDE and our KpLptDE, reveals a 21 degrees rotation of the LptD N-terminal domain that may impart flexibility on the trans-envelope LptCAD scaffold. Utilizingmutagenesis coupled to an in vivo functional assay and molecular dynamics simulations, we demonstrate the critical role of Pro231 and Pro246 in the function of the LptD lateral gate that allows partitioning of LPS into the outer membrane.
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