Journal
STEM CELL RESEARCH
Volume 17, Issue 2, Pages 342-351Publisher
ELSEVIER
DOI: 10.1016/j.scr.2016.07.010
Keywords
Mesenchymal stem cells; Adipose tissue; Platelet lysate angiogenesis; Transmission electron microscopy
Funding
- Sapienza University of Rome [C26A1429M2]
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Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived frommediastinal and subcutaneous depots (thoracic compartment) bymeans of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combinedwith lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed. (C) 2016 The Authors. Published by Elsevier B. V. This is an open access article under the CC BY-NC-ND license.
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