Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 105, Issue -, Pages 218-225Publisher
ELSEVIER
DOI: 10.1016/j.sajb.2016.04.003
Keywords
Antioxidant enzymes; Cell death; Glycine betaine; Hydrogen peroxide; Nitric oxide; Nitric oxide synthase; Lipid peroxidation
Categories
Ask authors/readers for more resources
Compatible solutes, such as glycine betaine (GB), are involved in improving plant tolerance to abiotic stress. In this study, we investigated the effects of exogenously applied nitric oxide (NO) donor 2,2'-(hydroxynitrosohydrazono) bis-ethanimine and nitric oxide synthase (NOS) inhibitor N-omega-Nitro-L-Arginine methyl ester (L-NAME) on GB content and its influence on ascorbate peroxidase (APX) enzymatic activity in roots and leaves of maize seedlings. Application of L-NAME (2 mM L-NAME or in combination with 200 mu M DETA) significantly increased cell death, H2O2 content, and lipid peroxidation but reduced GB content and APX activity. The effects of L-NAME treatment on maize were reversed by application of the NO donor 2, 2'-(hydroxynitrosohydrazono) bis-ethanimine (DETA/NO). Application of the NO donor to plants treated with L-NAME reversed the effects of L-NAME on GB content and APX activity, which were increased to levels higher than those in plants treated with L-NAME alone. These results show that exogenous application of the NOS inhibitor reduces APX activity and GB accumulation. Our data suggest that NOS activity plays a role in regulating the antioxidant defense mechanism and osmoprotection in plants. (C) 2016 SAAB. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available