Journal
SMALL
Volume 12, Issue 19, Pages 2634-2640Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201503934
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Funding
- Center for DNA Nanotechnology (CDNA)
- Danish National Research Foundation [DNRF81]
- Aarhus University, Graduate School of Science and Technology
- Villum Fonden [00007206] Funding Source: researchfish
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DNA origami provides rapid access to easily functionalized, nanometer-sized structures making it an intriguing platform for the development of defined drug delivery and sensor systems. Low cellular uptake of DNA nanostructures is a major obstacle in the development of DNA-based delivery platforms. Herein, significant strong increase in cellular uptake in an established cancer cell line by modifying a planar DNA origami structure with the iron transport protein transferrin (Tf) is demonstrated. A variable number of Tf molecules are coupled to the origami structure using a DNA-directed, site-selective labeling technique to retain ligand functionality. A combination of confocal fluorescence microscopy and quantitative (qPCR) techniques shows up to 22-fold increased cytoplasmic uptake compared to unmodified structures and with an efficiency that correlates to the number of transferrin molecules on the origami surface.
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