Journal
SMALL
Volume 12, Issue 28, Pages 3794-3801Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201601066
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Funding
- National Basic Research Program of China [2012CB933301]
- National Natural Science Foundation of China [21305070, 21475064]
- Natural Science Foundation of Jiangsu Province [BK20130861]
- Sci-tech Support Plan of Jiangsu Province [BE2014719]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R37]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [YX03001]
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The performance for biomolecular detection is closely associated with the interfacial structure of a biosensor, which profoundly affects both thermodynamics and kinetics of the assembly, binding and signal transduction of biomolecules. Herein, it is reported on a one-step and template-free on-electrode synthesis method for making shape-controlled gold nanostructures on indium tin oxide substrates, which provide an electrochemical sensing platform for ultrasensitive detection of nucleic acids. Thus-prepared hierarchical flower-like gold nanostructures (HFGNs) possess large surface area that can readily accommodate the assembly of DNA probes for subsequent hybridization detection. It is found that the sensitivity for electrochemical DNA sensing is critically dependent on the morphology of HFGNs. By using this new strategy, a highly sensitive electrochemical biosensor is developed for label-free detection of microRNA-21 (miRNA-21), a biomarker for lung cancers. Importantly, it is demonstrated that this biosensor can be employed to measure the miRNA-21 expression level from human lung cancer cell (A549) lysates and worked well in 100% serum, suggesting its potential for applications in clinical diagnosis and a wide range of bioanalysis.
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