Journal
SHOCK
Volume 45, Issue 6, Pages 641-646Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SHK.0000000000000548
Keywords
ALI; bone marrow mesenchymal stem cell; multiple trauma; NF-kappa B; toll-like receptor
Funding
- Zhejiang Province Natural Science Foundation of China [LY12H15001]
- Wenzhou Municipal Science and Technology Bureau project [S20090038]
- Health and Family Planning Commission of Zhejiang Province [2013KYA130]
- Science Technology Department of Zhejiang Province [2011R50018-20]
- National Natural Science Foundation of China [81171787]
Ask authors/readers for more resources
Background: Multiple trauma normally leads to acute lung injury (ALI) and other multiple organ dysfunction syndrome (MODS). Finding effective treatments for ALI remains a medical as well as socioeconomic challenge. Several studies show that bone marrow mesenchymal stem cells (BMSCs) have the potent anti-inflammation activity and transfusion of BMSCs can effectively inhibit inflammatory and autoimmune diseases. Methods: In this study, we investigated the TLR2, 4/NF-kappa B signaling pathway to determine the therapeutic value of BMSCs on lipopolysaccharide (LPS)-induced ALI. To investigate the immunosuppression effects of BMSCs, rats subjected to multiple trauma were administrated with LPS to induce ALI and then treated with BMSCs. The histology of the lung was examined. Serum levels of the pro-inflammatory factors TNF alpha, interleukin (IL)-6, and IL-1 beta, as well as anti-inflammatory factor IL-10 were measured at 3, 6, 12, and 24 h after the treatment. Moreover, expressions of TLR2 and TLR4 at the mRNA and protein levels, as well as phosphorylation of p65 in the lungs, were assessed at these time points. Results: We found that BMSCs reduced inflammatory injury, inhibited LPS-induced upregulation of TLR2 and TLR4 expression at the mRNA and protein levels, and compromised p65 phosphorylation. In addition, infusion of BMSCs also downregulated the abundance of pro-inflammatory TNF alpha, IL-6, and IL-1 beta and upregulated the abundance of anti-inflammatory IL-10 levels in the serum. Conclusions: Our results suggest that BMSCs suppress the inflammatory reactions through inhibition of the TLR2, 4 mediated NF-kappa B signal pathway, which hints that BMSCs can potentially be used to treat ALI in multiple trauma.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available