Identifying multiple bacterial pathogens by loop-mediated isothermal amplification on a rotate & react slipchip

A rotate & react SlipChip (RnR-SlipChip) was developed for simultaneous visual detection of multiple bacterial pathogens by loop-mediated isothermal amplification (LAMP). The SlipChip system consists of two round PDMS-glass hybrid chips that are assembled coaxially by a plastic screw-nut suite. After sample loading, one-step rotation allowed immediate mixing and reaction of multiple bacteria samples with LAMP reagents on the chip. A home-built peltier heating device was used to implement the isothermal control by an advanced RISC machines (ARM) processor. Under the optimized LAMP conditions, a fluorescent signal-to-noise ratio of about 5-fold and a detection limit of 7.2 copies/pi genomic DNA were achieved. Furthermore, five common digestive bacterial pathogens including Bacillus cereus, Escherichia coli, Salmonella enterica, Vibrio fluvialis and Vibrio parahaemolyticus, were visually identified using the established method in 60 min with a success rate of 100%. The developed RnR-SlipChip provided a rapid, simple-to-use and cost-effective solution for pathogenic bacteria detection, which holds high potential for clinical diagnosis especially in resource-limited areas. (C) 2016 Elsevier B.V. All rights reserved.