Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 233, Issue -, Pages 289-297Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.04.091
Keywords
Exosome; Purification; Separation; Nanoporous membrane; Body fluid
Funding
- KIST institutional program [2E25590]
- National Research Foundation of Korea (NRF) grant - Korean government (MSIP) [2011-0030075, 2011-0028845]
- Ministry of Science, ICT & Future Planning, Republic of Korea [2E25590] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We present a high-yield electro-migration method to isolate extracellular vesicles (EVs), which achieves ultracentrifugation (U/C)-level size exclusion from biological fluids (e.g.,plasma).An electric field applied across a dialysis membrane with an appropriate pore size (30nm), facilitates protein migration through the membrane, but captures EVs on the membrane surface. Compared to conventional procedures, this method shows up to 65% EV recovery estimated at the RNA level (similar to 7.9 times better than U/C) and up to 83.6% protein removal (residual protein amount is approximately half of the precipitate) in similar to 30 min (similar to 9 times faster than U/C). With optimized working range of voltage and use of biochemically stable buffers, isolated EV are fully compatible with biological post-processes and assays. (C) 2016 Elsevier B.V. All rights reserved.
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