Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 223, Issue -, Pages 861-867Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.09.121
Keywords
Hairpin-like DNA; Gold nanoparticles; Electrochemical biosensor; Electrochemical impedance spectra
Funding
- National Natural Science Foundation of China [21275127]
- Program for New Century Excellent Talents in Fujian Province University [JA12204]
- Education Department of Fujian Province [JA14195]
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An ultrasensitive electrochemical DNA biosensor has been developed based on the assembly of highly conductive gold nanoparticles (AuNPs) on the free terminal of hairpin-structured probe DNA. The hairpin probe DNA was first immobilized on a gold electrode surface through Au-S bond. Then the thioglycolic acid (TGA) was grafted on the T-end of hairpin DNA via the carboxylic-amino condensation reaction. Followed by, the AuNPs were further attached through reaction with the thiol-group of TGA. Thus, a highly conductive biointerface with ultralow charge-transfer resistance was obtained. Quantitative analysis exhibited that the target DNA could be detected over the range from 1.0 x 10(-17) to 1.0 x 10(-11) M. The detection limit was estimated to be 1.7 x 10(-18) M. Also the biosensor showed good hybridization selectivity, stability and regeneration ability. (C) 2015 Elsevier B.V. All rights reserved.
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