Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 233, Issue -, Pages 307-313Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.04.089
Keywords
Thiols; Detection; Fluorescent probe; Bioimaging
Funding
- National Natural Science Foundation of China [21472118]
- Program for the Top Young and Middle-aged Innovative Talents of Higher Learning Institutions of Shanxi [2013802]
- Talents Support Program of Shanxi Province [2014401]
- Shanxi Province Outstanding Youth Fund [2014021002]
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Herein we report a highly selective thiol-reactive fluorescent probe based on a nucleophilic substitution reaction for detecting thiols over other relevant biological species. The probe, triphenylamine as a fluorophore and 2,4-dinitrobenzenesulfonyl chloride as a nucleophilic substitution group was synthesized and characterized. The ability to specially recognizing thiol was investigated by UV-vis and fluorescence spectrometers. Among the tested amino acids, only Cys, Hcy and GSH could turn on the fluorescence emission. It suggested that the system was a highly selective sensor for thiols. More importantly, the compound features a good linearity range and the detection limit is as low as 10(-8) mol/L. In addition, as a typical biological thiol, the ability of probe to detecting Cys in living cells (HepG2 cells) via an enhancement of the fluorescence was proved. (C) 2016 Elsevier B.V. All rights reserved.
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