Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 233, Issue -, Pages 691-696Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.04.144
Keywords
Hybridization chain reaction; Fluorescence resonance energy transfer; DNA; Ratiometric measurement
Funding
- NSFC [21177023]
- Key Project of Chinese Ministry of Education [211084]
- Science Foundation of Fujian Province of China [2013101044]
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A versatile enzyme-free deoxyribonucleic acid (DNA) detection method based on hybridization chain reaction (HCR) and fluorescence resonance energy transfer (FRET) was developed. Hairpin H1 and H2 are labelled with carboxyfluorescein (FAM) as the donor and tetramethylrhodamine (TAMRA) as the acceptor, respectively. In the presence of target DNA, long nicked dsDNA nanowires are self-assembled through HCR process. The donor and the acceptor are brought in close proximity, resulting in a FRET process between them. The ratio of fluorescent intensities of the acceptor and donor can be used to quantitatively detect the target DNA with a limit of detection 0.7 nmol L-1. This proposed method was applied to detect DNA in biologic samples with satisfactory results. (C) 2016 Elsevier B.V. All rights reserved.
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