Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 235, Issue -, Pages 563-567Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2016.05.118
Keywords
Colorimetric; Botulinum neurotoxin; Gold nanoparticles
Funding
- National Natural Science Foundation of China [51305375]
- City University of Hong Kong [6987032]
- Hong Kong Research Grant Council [21214815]
- Science Technology and Innovation Committee of Shenzhen Municipality [JCYJ20150601102053061]
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We report a colorimetric detection of functional botulinum neurotoxin (BoNT) based on its proteolyticactivity. Active BoNT can enzymatically cleave SNAptide, a short peptide with 15 amino acids. The cleaved peptides subsequently induced the agglomeration of gold nanoparticles, allowing a colorimetric signal that can be discerned by the naked eye at the low concentration of 1 ng/mL (6.67 pM), and achieve low limit of detection at 0.25 ng/mL (1.67 pM 3 sigma/s) by spectral absorbance. This colorimetric detection will provide a simple and promising method for applications at low-resource sites. (C) 2016 Elsevier B.V. All rights reserved.
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