Journal
SENSORS
Volume 16, Issue 9, Pages -Publisher
MDPI AG
DOI: 10.3390/s16091488
Keywords
fluorescence resonance energy transfer (FRET); biosensors; fluorescent proteins
Funding
- US National Institutes of Health [GM08042]
- NIH [1U01NS090600, P50GM107615]
- Rita Allen Foundation Scholar Award
- Shenzhen Science and Technology Innovation grant [JCYJ20150521144320987]
- Hundred Talents Program Award of the Chinese Academy of Sciences [Y64401]
- National Natural Science Foundation of China [1431670872]
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Forster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies.
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