Journal
SENSORS
Volume 16, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/s16050630
Keywords
nitrogen-doped carbon dot; glucose oxidase; enzyme immobilization; biosensor; electrochemical detection
Funding
- National Natural Science Foundation of China [21104032]
- Natural Science Foundation of Jiangsu Province [BK2011413]
- Fundamental Research Funds for the Central Universities [1104020505]
- Program for Changjiang Scholars and Innovative Research Team in University
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Nitrogen-doped carbon dots are introduced as a novel substrate suitable for enzyme immobilization in electrochemical detection metods. Nitrogen-doped carbon dots are easily synthesised from polyacrylamide in just one step. With the help of the amino group on chitosan, glucose oxidase is immobilized on nitrogen-doped carbon dots-modified carbon glassy electrodes by amino-carboxyl reactions. The nitrogen-induced charge delocalization at nitrogen-doped carbon dots can enhance the electrocatalytic activity toward the reduction of O-2. The specific amino-carboxyl reaction provides strong and stable immobilization of GOx on electrodes. The developed biosensor responds efficiently to the presence of glucose in serum samples over the concentration range from 1 to 12 mM with a detection limit of 0.25 mM. This novel biosensor has good reproducibility and stability, and is highly selective for glucose determination under physiological conditions. These results indicate that N-doped quantum dots represent a novel candidate material for the construction of electrochemical biosensors.
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