Journal
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
Volume 55, Issue -, Pages 148-155Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.semcdb.2015.11.001
Keywords
Mouse embryo; Light-sheet; Microscopy; Live-imaging
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Funding
- European Molecular Biology Laboratory
- Center of Modeling and Simulation in the Biosciences (BIOMS) of the University of Heidelberg
- EMBL International PhD Programme
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Tackling modern cell and developmental biology questions requires fast 3D imaging with sub-cellular resolution over extended periods of time. Fluorescence microscopy has emerged as a powerful tool to image biological samples with high spatial and temporal resolution with molecular specificity. In particular, the highly efficient illumination and detection scheme of light-sheet fluorescence microscopy is starting to revolutionize the way we can monitor cellular and developmental processes in vivo. Here we summarize the state-of-the art of light-sheet imaging with a focus on mammalian development - from instrumentation, mounting and sample handling to data processing. (C) 2016 Published by Elsevier Ltd.
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