Journal
SCIENCE CHINA-LIFE SCIENCES
Volume 59, Issue 2, Pages 213-218Publisher
SCIENCE PRESS
DOI: 10.1007/s11427-016-5004-y
Keywords
UDP-glucose dehydrogenase; UDP-GlcA; Gossypium; fiber
Categories
Funding
- Ministry of Agriculture of China [2014ZX08009-003]
- Hebei Province Technology Support Program [14962905D]
- Hebei Province Department of Education Fund [Y2012025]
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Uridine diphosphate-glucose dehydrogenase (UGD, EC1.1.1.22 oxidizes UDP-Glc (UDP-D-glucose) to UDP-GlcA (UDP-D-glucuronate), a critical precursor of cell wall polysaccharides. GbUGD6 from Gossypium barbadense is more highly expressed late in the elongation of cotton fibers (15 d post-anthesis (DPA)) and during the stage of secondary cell wall thickening (30 DPA). Subcellular localization analysis in onion epidermis revealed that fluorescently labeled GbUGD6 protein was distributed throughout the cell membrane, as well as the nucleus and vacuoles. Examination of UGD function in Arabidopsis revealed that the antisense GbUGD6 lines had shorter roots, deferred blossoming, compared to wild-type plants. Activities of associated enzymes were also affected by UGD reduction, and biochemical analysis of cell wall samples showed an increase in cellulose levels and a decrease in UGP-GlcA contents. The results of the present study as well as previous studies on UGD support the conclusion that UGD plays a major role in synthesizing polysaccharides synthesis in the cell wall.
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