Journal
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
Volume 17, Issue 1, Pages 677-684Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14686996.2016.1240006
Keywords
Poly(ethylene glycol)-conjugated phospholipid (PEG-lipids); supported lipid membrane; surface modification; membrane fluidity; hydrophobic interaction
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [2306, 21240051, 21650118]
- Grants-in-Aid for Scientific Research [16H01692] Funding Source: KAKEN
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We studied real-time interaction between poly(ethylene glycol)-conjugated phospholipids (PEG-lipids) and a supported lipid membrane by surface plasmon resonance (SPR) spectroscopy to understand dynamic behaviors of PEG-lipids on living cell membranes. Supported lipid membranes formed on a hydrophobic surface were employed as a model of living cell membrane. We prepared three kinds of PEG-lipids that carried alkyl chains of different lengths for SPR measurements and also performed fluorescence recovery after photobleaching (FRAP) to study the influence of acyl chain length on dynamics on the supported membrane. PEG-lipids were uniformly anchored to lipid membranes with high fluidity without clustering. Incorporation and dissociation rates of PEG-lipids into supported membranes strongly depended on the length of acyl chains; longer acyl chains reduced the incorporation rate and the dissociation rate of PEG-lipid. Furthermore, protein adsorption experiment with bovine serum albumin indicated that PEG modification prevented the adsorption of bovine serum albumin on such supported membrane. [GRAPHICS] .
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