Journal
SCIENCE
Volume 352, Issue 6285, Pages 595-599Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aad9964
Keywords
-
Categories
Funding
- HHMI's Collaborative Innovation Awards
- NIH [R01-GM56322]
- Welch Foundation [I-1544]
- Cancer Research Institute Irvington postdoctoral fellowship
- National Research Service Award [F32 (5-F32-DK101188)]
- Tobacco-Related Disease Research Program of the University of California [19FT-0090]
Ask authors/readers for more resources
Activation of various cell surface receptors triggers the reorganization of downstream signaling molecules into micrometer-or submicrometer-sized clusters. However, the functional consequences of such clustering have been unclear. We biochemically reconstituted a 12-component signaling pathway on model membranes, beginning with T cell receptor (TCR) activation and ending with actin assembly. When TCR phosphorylation was triggered, downstream signaling proteins spontaneously separated into liquid-like clusters that promoted signaling outputs both in vitro and in human Jurkat T cells. Reconstituted clusters were enriched in kinases but excluded phosphatases and enhanced actin filament assembly by recruiting and organizing actin regulators. These results demonstrate that protein phase separation can create a distinct physical and biochemical compartment that facilitates signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available