Journal
SCIENCE
Volume 352, Issue 6286, Pages 687-690Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aad8036
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Funding
- NIH [R01GM073960, R01GM117968, GM094597]
- Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy [W-31-109-Eng-38]
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Natural recombination combines pieces of preexisting proteins to create new tertiary structures and functions. We describe a computational protocol, called SEWING, which is inspired by this process and builds new proteins from connected or disconnected pieces of existing structures. Helical proteins designed with SEWING contain structural features absent from other de novo designed proteins and, in some cases, remain folded at more than 100C. High-resolution structures of the designed proteins CA01 and DA05R1 were solved by x-ray crystallography (2.2 angstrom resolution) and nuclear magnetic resonance, respectively, and there was excellent agreement with the design models. This method provides a new strategy to rapidly create large numbers of diverse and designable protein scaffolds.
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