Journal
SCIENCE
Volume 351, Issue 6276, Pages 969-972Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aad8857
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Funding
- European Molecular Biology Organization
- Human Frontier Science Program
- Weizmann Institute Women in Science Program
- Deutsche Forschungsgemeinschaft [FO 716/4]
- Center for Integrated Protein Science Munich
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The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.
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