Journal
SCIENCE
Volume 352, Issue 6283, Pages 363-365Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf0643
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Funding
- EMBL's Electron Microscopy and Proteomics Core Facilities
- EMBL Interdisciplinary Postdoc Programme under Marie Curie COFUND actions
- Robert Crooks Stanley Fellowship at the Stevens Institute of Technology and National Institute on Aging (NIA) [1R21AG047433-01]
- Ignition Grant Initiative from Stevens Institute of Technology
- NIA [1R21AG047433-01]
- EMBL
- European Research Council [309271-NPCAtlas]
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Nuclear pore complexes (NPCs) are 110-megadalton assemblies that mediate nucleocytoplasmic transport. NPCs are built from multiple copies of similar to 30 different nucleoporins, and understanding how these nucleoporins assemble into the NPC scaffold imposes a formidable challenge. Recently, it has been shown how the Y complex, a prominent NPC module, forms the outer rings of the nuclear pore. However, the organization of the inner ring has remained unknown until now. We used molecular modeling combined with cross-linking mass spectrometry and cryo-electron tomography to obtain a composite structure of the inner ring. This architectural map explains the vast majority of the electron density of the scaffold. We conclude that despite obvious differences in morphology and composition, the higher-order structure of the inner and outer rings is unexpectedly similar.
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