Journal
SCIENCE
Volume 354, Issue 6315, Pages 1036-1041Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf6136
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Funding
- Japan Society for the Promotion of Science KAKENHI [25111005, JP16H06280]
- Grants-in-Aid for Scientific Research [15H01388, 16H06280, 25111005] Funding Source: KAKEN
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In macroautophagy, cytoplasmic contents are sequestered into the double-membrane autophagosome, which fuses with the lysosome to become the autolysosome. It has been thought that the autophagy-related (ATG) conjugation systems are required for autophagosome formation. Here, we found that autophagosomal soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) syntaxin 17-positive autophagosome-like structures could be generated even in the absence of the ATG conjugation systems, although at a reduced rate. These syntaxin 17-positive structures could further fuse with lysosomes, but degradation of the inner autophagosomal membrane was significantly delayed. Accordingly, autophagic activity in ATG conjugation-deficient cells was strongly suppressed. We suggest that the ATG conjugation systems, which are likely required for the closure (i.e., fission) of the autophagosomal edge, are not absolutely essential for autolysosome formation but are important for efficient degradation of the inner autophagosomal membrane.
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