Journal
RNA
Volume 22, Issue 5, Pages 657-659Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.056325.116
Keywords
MS2 RNA; MS2-MCP system; RNA decay; yeast; mRNA localization
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Funding
- NIGMS NIH HHS [R01 GM045443] Funding Source: Medline
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The binding of MS2-GFP protein to arrays of MS2 sites in yeast mRNAs has been used extensively to visualize mRNA localization. We previously reported that arrays of MS2 sites bound by MS2 protein could inhibit Xrn1p and lead to the accumulation of 3' mRNA decay fragments. We suggest that these decay fragments have the potential to complicate mRNA localization studies, as stated in an earlier study.
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