Journal
RNA
Volume 22, Issue 5, Pages 660-666Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.055095.115
Keywords
MS2 aptamer; MS2 coat protein; mRNA localization; mRNA decay; P-bodies
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Funding
- Dean of Faculty and Sir Charles Clore postdoctoral fellowship
- German-Israel Foundation (GIF), Germany [G-1003-122.13/2008, I-1190-96.13/2012]
- Minerva Foundation, Germany
- National Institutes of Health [NHGRI U54HG00306]
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The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3' decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.
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