Journal
RNA BIOLOGY
Volume 14, Issue 5, Pages 603-610Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1184387
Keywords
Cytidine deaminase; disease genes; epitranscriptomics; RNA editing; RNA seq
Categories
Funding
- Department of Pathology of Roswell Park Cancer Institute
- Department of Thoracic Surgery of Roswell Park Cancer Institute
- Cancer Center of National Cancer Institute [5P30 CA016056]
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APOBEC3A cytidine deaminase induces site-specific C-to-U RNA editing of hundreds of genes in monocytes exposed to hypoxia and/or interferons and in pro-inflammatory macrophages. To examine the impact of APOBEC3A overexpression, we transiently expressed APOBEC3A in HEK293T cell line and performed RNA sequencing. APOBEC3A overexpression induces C-to-U editing at more than 4,200 sites in transcripts of 3,078 genes resulting in protein recoding of 1,110 genes. We validate recoding RNA editing of genes associated with breast cancer, hematologic neoplasms, amyotrophic lateral sclerosis, Alzheimer disease and primary pulmonary hypertension. These results highlight the fundamental impact of APOBEC3A overexpression on human transcriptome by widespread RNA editing.
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