Journal
REVISTA ARGENTINA DE MICROBIOLOGIA
Volume 48, Issue 2, Pages 137-142Publisher
ASOCIACION ARGENTINA MICROBIOLOGIA
DOI: 10.1016/j.ram.2016.03.003
Keywords
Cryptococcus neoformans; Fluconazole resistance; ERG11 gene; Amino acid substitution; Mutation
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The molecular basis of fluconazole resistance in Cryptococcus neoformans has been poorly studied. A common azole resistance mechanism in Candida species is the acquisition of point mutations in the ERG11 gene encoding the enzyme lanosterol 14-alpha-demethylase, target of the azole class of drugs. In C. neoformans only two mutations were described in this gene. In order to evaluate other mutations that could be implicated in fluconazole resistance in C. neoformans we studied the genomic sequence of the ERG11 gene in 11 clinical isolates with minimal inhibitory concentration (MIC) values to fluconazole of = 16 mu g/ml. The sequencing revealed the G1855A mutation in 3 isolates, resulting in the enzyme amino acid substitution G484S. These strains were isolated from two fluconazole-treated patients. This mutation would not intervene in the susceptibility to itraconazole and voriconazole. (C) 2016 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U.
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