Journal
RESEARCH ON CHEMICAL INTERMEDIATES
Volume 43, Issue 4, Pages 2415-2432Publisher
SPRINGER
DOI: 10.1007/s11164-016-2770-x
Keywords
RT-PCR; SDS-PAGE; Antioxidant enzymes; Adventitious roots; HPLC-ESI-MSn
Categories
Funding
- 863 Program [2014AA022201-04]
- ministry of education new teacher fund, China [20130032120084]
- Tianjin Youth Fund [15JCQNJC13300]
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In this study, Candida tropicalis, Aspergillus niger, and a mixture of their cultures were previously used as fungal elicitors in Panax quinquefolium adventitious root culture to enhance ginsenoside production. The result showed that ginsenoside content (11.47 +/- 0.54 mg g(-1)) was significantly enhanced to 3.25-fold of the control group after treatment with 400 mg L-1 C. tropicalis. The polysaccharide content (213.01 +/- 18.63 mg g(-1)) reached the highest when A. niger was at the concentration of 200 mg L-1. HPLC-ESI-MSn analysis was performed, showing that ginsenosides Rh-1 and Ro were identified after treatment with fungal elicitors. Furthermore, we found that C. tropicalis, A. niger, and mix elicitor significantly up-regulated the expression of the squalene synthase, squalene epoxidase, dammarenediol synthase, beta-amyrin synthase, protopanaxadiol synthase, protopanaxatriol synthase, and UDP-glycosyltransferase. SDS-polyacrylamide gel electrophoresis was used to analysis the changes of protein. A new band was discovered at the size of 75 kDa. In addition, it found that fungal elicitors significantly increased the activities of catalases and peroxidase.
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