Journal
REPRODUCTIVE TOXICOLOGY
Volume 64, Issue -, Pages 169-179Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2016.06.001
Keywords
Embryoid bodies; Progesterone; Calcium; Cardiomyocyte; Cardiomyogenesis
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Progesterone (PG) and its derivates are used in prevention of spontaneous miscarriage. However, some studies have reported that exposure to PG and its derivates during pregnancy can cause malformations and affect both blood pressure and the cardiovascular system. The effect of PG on cardiomyogenesis of mouse embryonic stem cells (mESCs) is not well known. Expression of Pgr mRNA showed an opposite pattern of beating-ratio during differentiation. PG treatment resulted in reduction of the beating ratio to 60.45 +/- 1.54% from 92.17 +/- 2.98% in normal differentiation, reduced transcripts of heart morphogenesis and Ca2+ binding-related genes in the next generation sequencing data and significantly decreased expression levels of Ca2+/contraction-related genes including Ryr2, Calm2, Trpv2, and Mylk3, the intracellular Ca2+ level, and the beating frequency. These results suggest that PG exerts inhibitory effects on differentiation of mESCs into functional cardiomyocytes. (C) 2016 Elsevier Inc. All rights reserved.
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