Journal
REPRODUCTIVE BIOLOGY
Volume 16, Issue 1, Pages 61-69Publisher
INST ANIMAL REPRODUCTION FOOD RESEARCH
DOI: 10.1016/j.repbio.2015.11.002
Keywords
In vitro oocyte maturation; Oviducts; Coculture techniques; Zona pellucida; In vitro fertilization
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Funding
- Iran National Science Foundation [93020720]
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The central role of the oviduct, as the site of zona pellucida (ZP) maturation, fertilization and early embryogenesis, has been recognized. The objective of this study was to investigate whether ampullary and isthmic derived epithelial cells have different effects on in vitro ZP hardening, in vitro fertilization (IVF) and in vitro culture (IVC) of the resulting embryos. Cumulus oocyte complexes (COCs) were matured in a coculture system with ampullary/isthmic epithelial cells, TCM199 supplemented with insulin-like growth factor I (IGF-I) and epithelial derived growth factor (EGF) (GF treated group), conditioned media produced using ampullary (ACM), isthmic (ICM), COCs + ampullary, and COCs + isthmic epithelial cells, contactless culture system, oviductal fluid, GF + ACM/ICM, and drops of TCM199 (control), for 24 h. The matured oocytes were randomly divided into two groups: Group I was subjected to ZP digestion; Group II underwent IVF. The duration of the ZP digestion, in a coculture system with ampullary epithelial cells (AE) was significantly increased (p < 0.05), compared with other groups. Penetrated oocytes and monospermic fertilization were significantly increased (p < 0.05) in the AE group. The mean number of spermatozoa per penetrated oocyte was reduced dramatically for the AE group (p < 0.05). A significant increase (p < 0.05) in the embryo development was observed in all treated groups, compared to the control. Results revealed that epithelial cells harvested from the ampullary segment of the oviduct had in vitro specialized role in ZP hardening and have subsequent IVF and IVC outcomes. (C) 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z o.o. All rights reserved.
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