4.3 Article

Creation of DNA aptamers against recombinant bone morphogenetic protein 15

Journal

REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 28, Issue 8, Pages 1164-1171

Publisher

CSIRO PUBLISHING
DOI: 10.1071/RD14409

Keywords

antibody; evolution; mutation; Systematic Evolution of Ligands by EXponential enrichment (SELEX)

Funding

  1. National Natural Science Foundation [81270734, 31200979]
  2. Ministry of Science and Technology of China [2011DFG33320]
  3. Ministry of Science and Innovation of New Zealand [C10X1012]
  4. New Zealand Ministry of Business, Innovation & Employment (MBIE) [C10X1012] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)

Ask authors/readers for more resources

The oocyte-derived growth factor bone morphogenetic protein (BMP) 15 plays important roles in fertility, but its mechanism of action differs between species. Generation of BMP15-binding molecules, as an essential investigation tool, would be helpful to provide valuable insight into the underlying biological features of BMP15. The BMP15-binding molecules could be antibodies or aptamers. Aptamers have many advantages over antibodies as macromolecular ligands for target proteins. DNA aptamers can be obtained by a method of Systematic Evolution of Ligands by EXponential enrichment (SELEX) beginning with a pool of random sequences. However, the success of this technique cannot be guaranteed if the initial pool lacks candidate sequences. Herein, we report on the creation of DNA aptamers by means of modified SELEX. The modification included enhanced mutation and progressive selection during an in vitro evolutionary process. As a proof-of-principle, we started from a single sequence instead of a multiple-sequence pool. Functional aptamers against the recombinant BMP15 were successfully created and identified.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available