Journal
RENAL FAILURE
Volume 38, Issue 6, Pages 945-951Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/0886022X.2016.1164185
Keywords
Inflammation; kidney fibrosis; oxymatrine; TGF-; Smad3
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Funding
- Natural Science Foundation of Hubei Province [2014CFC1110, WJ2015Z028]
- Agricultural and Social Science Development Plan of Enshi State in Hubei Province
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This study investigated whether oxymatrine (OMT) treatment can ameliorate renal interstitial fibrosis in unilateral ureteral obstruction (UUO) mice model. Moreover, the potential mechanisms of such treatment were analyzed. Twenty-four C57/BL6 mice were randomly divided into three groups, namely sham group, vehicle plus unilateral ureteral obstruction (UUO)-treated group, and 100mg/kg/d OMT plus UUO-treated group. All mice were euthanized seven days after surgery, and their kidneys were harvested. Renal injury, fibrosis, expression of proinflammatory cytokines, and the transforming growth factor-1/Smads (TGF-/Smads) and nuclear factor-kappa B (NF-B)-signaling pathways were assessed. The results showed OMT significantly prevented kidney injury and fibrosis, as evidenced by decreased expression of collagen-1 and fibronectin. Furthermore, OMT administration inhibited the release of inflammatory factors including tumor necrosis factor-, (TNF-) interleukin-1 (IL-1), and interleukin-6 (IL-6), as well as phosphorylated NF-B p65. In addition, OMT blocked the activation of myofibroblasts by inhibiting the TGF-/Smad3-signaling pathway. The findings indicate that OMT-attenuated renal fibrosis and inflammation, and this renoprotective effect may be ascribed to the inactivation of the TGF-/Smad3 and NF-B p65 pathways.
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