Journal
PROTEOMICS CLINICAL APPLICATIONS
Volume 10, Issue 9-10, Pages 1068-1072Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.201600069
Keywords
Benchmark proteome; Clostridium difficile; Comprehensive peptide dataset; Label-free protein quantification; Voronoi treemaps
Funding
- Federal State of Lower Saxony
- Niedersachsisches Vorab [VWZN2889]
- Federal State of Mecklenburg-Vorpommern [UG 14 001]
Ask authors/readers for more resources
The intestinal pathogen Clostridium difficile is causing an increasing number of infections often characterized by severity and high relapse rates. Profound knowledge of the physiology of the pathogen could help to develop new treatment strategies. Proteomics, a valuable tool to study bacterial physiology, was used in this work to establish a benchmark proteome of reference strain C. difficile 630 Delta erm with MS-based details on all identified proteins. Our elaborate annotation and visualization of C. difficile 630 Delta erm 3764 ORFs will serve as a valuable base for researchers having to evaluate global expression studies. To exemplify expression variability, protein expression of late exponentially growing cells in complex brain-heart infusion medium and C. difficile minimal medium was compared. Noteworthy results of this comparison are as follows: (i) the higher expression of enzymes for the biosynthesis of some vitamins and purine and (ii) downregulation of proteins involved in butanoate fermentation in C. difficile minimal medium. However, the abundance of proteins involved in DNA metabolism, protein synthesis, and the cell envelope showed no variation between the two growth media.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available