Journal
PROTEOMICS
Volume 16, Issue 8, Pages 1291-1296Publisher
WILEY
DOI: 10.1002/pmic.201500412
Keywords
Cell free ribosome display; Dot blot screening; Multiple antibody generation in vitro; Protein arrays
Funding
- 863 program, China [2006AA02 Z162]
- Chengdu Institute of Biological Products, SinoPharm. China
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In vitro display technology is a powerful tool for discovery and optimisation of novel antibodies. With increasing demands on various binding molecules in proteomics studies, techniques for a large-scale generation of antibodies or antibody fragments are needed. Here, we describe a novel method for parallel generation of different antibody fragments (scFv) by integrating cell-free ribosome display with array technology. We have demonstrated the procedure by successfully isolating scFv antibodies specific to 16 different cancer biomarkers via a single process. Our results provide proof of principle for multiple production of various scFv antibodies simultaneously.
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