Journal
PROSTAGLANDINS & OTHER LIPID MEDIATORS
Volume 123, Issue -, Pages 1-8Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.prostaglandins.2016.02.001
Keywords
3T3-L1; Obesity; Adipogenesis; 15-(S)-hydroxyeicosatetraenoic acid; PPAR gamma; hBMSC
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Funding
- Konkuk University
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Excess adipogenesis is a characteristic of obesity, which is associated with serious health problem, including type 2 diabetes. Here, to better understand the mechanisms for the development of adipocytes, we investigated the regulatory role of 15-(S)-hydroxyeicosatetraenoic acid (15(S)-HETE) in adipogenesis by treating 3T3-L1 murine preadipocytes and human bone marrow mesenchymal stem cells (hBMSCs) with 15(S)-HETE. In the 3T3-L1 study, 15(S)-HETE stimulated lipid accumulation and enhanced expression of adipogenic markers such as peroxisome proliferator-activated receptor gamma (PPAR gamma), yet reduced the activity of factor negatively controlling adipogenesis, such as the 5'-AMP-activated protein kinase. In the early stage of adipogenesis, 15(S)-HETE increased activation of protein kinase B and expression of CCAAT/enhancer-binding protein (C/EBP)beta and C/EBP delta. Finally, 15(S)-HETE promoted adipogenesis in hBMSCs, and resulted in increased lipid accumulation and expression of adipogenic markers. In conclusion, 15(S)-HETE functions as a natural PPAR gamma agonist and enhances adipogenesis. Our findings may provide the basis for the development of novel therapeutic measures to treat obesity. (C) 2016 Elsevier Inc. All rights reserved.
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