Journal
PROGRESS IN LIPID RESEARCH
Volume 63, Issue -, Pages 153-164Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.plipres.2016.06.001
Keywords
Biocatalysis; Lipase; Lipid modification; Phospholipase; P450 monooxygenase; Protein engineering
Funding
- European Union's Horizon research and innovation programme [635405]
- H2020 Societal Challenges Programme [635405] Funding Source: H2020 Societal Challenges Programme
Ask authors/readers for more resources
This review first provides a brief introduction into the most important tools and strategies for protein engineering (i.e. directed evolution and rational protein design combined with high-throughput screening methods) followed by examples from literature, in which enzymes have been optimized for biocatalytic applications. This covers engineered lipases with altered fatty acid chain length selectivity, fatty acid specificity and improved performance in esterification reactions. Furthermore, recent achievements reported for phospholipases, lipoxygenases, P450 monooxygenases, decarboxylating enzymes, fatty acid hydratases and the use of enzymes in cascade reactions are treated. (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available