Journal
PROCESS BIOCHEMISTRY
Volume 51, Issue 10, Pages 1413-1419Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2016.07.019
Keywords
Phasin; Cadaverine; In situ immobilization; Polyhydroxybutyrate
Categories
Funding
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2015R1A2A2A04006014, NRF-2015M1A5A1037196, NRF-2015R1D1A1A01060206]
- R&D Program of MOTIE/KEIT [10047910]
- Cooperative Research Program for Agriculture Science & Technology Development [010205022014]
- Energy Efficiency & Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant - the South Korean Government Ministry of Trade, Industry and Energy (MOTIE) [20133030000300]
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Cadaverine is a useful chemical that can be produced by lysine decarboxylase up to molar concentration levels. To develop a convenient and reusable production process, we performed intracellular immobilization of lysine decarboxylase (CadA) using poly(3-hydroxybutyrate) (P(3HB)) and PhaP1 (P(3HB) granule-associated protein) from Ralstonia eutropha. By adding 591 bp of the entire phaP1 gene sequence to the 3' end of the cadA gene, CadA was successfully fused to PhaP1. The phasin-fused CadA bound to the intracellular P(3HB) granules, which enabled the reuse of CadA in repetitive enzyme reactions. Although immobilization of the CadA-P(3HB) complex was not effective over extended temperature and pH ranges, the immobilized CadA exhibited increased thermal stability, with a half-life of 70 hat 50 degrees C. The CadA-P(3HB) complex achieved a 75-80% conversion yield over five reaction cycles without laborious immobilization steps. This study indicates the feasibility of in situ immobilization of lysine decarboxylase by phasin fusion. (C) 2016 Elsevier Ltd. All rights reserved.
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