Journal
PROCESS BIOCHEMISTRY
Volume 51, Issue 4, Pages 528-534Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2016.01.004
Keywords
Bromelain; Aqueous micellar two-phase system; Triton X-114; Ionic liquids; Co-surfactant; Purification
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Funding
- FEDER funds through the program COMPETE
- Portuguese Foundation for Science and Technology (FCT) [Pest-C/CTM/LA0011/2013, SFRH/BD/101683/2014, SFRH/BPD/79263/2011]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/23726-4]
- project FAPESP [2013\08617-7]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/101683/2014] Funding Source: FCT
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/23726-4] Funding Source: FAPESP
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Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved.
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