Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 10, Pages 2573-2578Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1523356113
Keywords
Paterno-Buchi reaction; glycerophospholipids; photochemical reaction; lipid biomarkers; cancerous tissue analysis
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Funding
- National Science Foundation [CHE-1308114]
- National Institutes of Health [1R01GM106016, NS073636]
- American Society for Mass Spectrometry research award
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1308114] Funding Source: National Science Foundation
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The field of lipidomics has been significantly advanced by mass spectrometric analysis. The distinction and quantitation of the unsaturated lipid isomers, however, remain a long-standing challenge. In this study, we have developed an analytical tool for both identification and quantitation of lipid C=C location isomers from complex mixtures using online Paterno-Buchi reaction coupled with tandem mass spectrometry (MS/MS). The potential of this method has been demonstrated with an implementation into shotgun lipid analysis of animal tissues. Among 96 of the unsaturated fatty acids and glycerophospholipids identified from rat brain tissue, 50% of them were found as mixtures of C=C location isomers; for the first time, to our knowledge, the quantitative information of lipid C=C isomers from a broad range of classes was obtained. This method also enabled facile cross-tissue examinations, which revealed significant changes in C=C location isomer compositions of a series of fatty acids and glycerophospholipid (GP) species between the normal and cancerous tissues.
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