Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 31, Pages E4567-E4576Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1604936113
Keywords
retrograde signaling; redox regulation; stress sensing; chloroplast; drought stress
Categories
Funding
- Australian Research Council Centre of Excellence in Plant Energy Biology [CE140100008]
- Wellcome Trust [092283]
- European Commission [625451]
- Endeavour International Postgraduate Research Scholarship (IPRS)
- ANU
- Medical Research Council [G116/172] Funding Source: researchfish
- MRC [G116/172] Funding Source: UKRI
Ask authors/readers for more resources
Intracellular signaling during oxidative stress is complex, with organelle-to-nucleus retrograde communication pathways ill-defined or incomplete. Here we identify the 3'-phosphoadenosine 5'-phosphate (PAP) phosphatase SAL1 as a previously unidentified and conserved oxidative stress sensor in plant chloroplasts. Arabidopsis thaliana SAL1 (AtSAL1) senses changes in photosynthetic redox poise, hydrogen peroxide, and superoxide concentrations in chloroplasts via redox regulatory mechanisms. AtSAL1 phosphatase activity is suppressed by dimerization, intramolecular disulfide formation, and glutathionylation, allowing accumulation of its substrate, PAP, a chloroplast stress retrograde signal that regulates expression of plastid redox associated nuclear genes (PRANGs). This redox regulation of SAL1 for activation of chloroplast signaling is conserved in the plant kingdom, and the plant protein has evolved enhanced redox sensitivity compared with its yeast ortholog. Our results indicate that in addition to sulfur metabolism, SAL1 orthologs have evolved secondary functions in oxidative stress sensing in the plant kingdom.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available