Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 35, Pages E5242-E5249Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1519555113
Keywords
osmotic sensing; calcium; salt stress; plastid; abscisic acid
Categories
Funding
- Life Sciences Research Foundation - US Department of Energy, Office of Science, Office of Basic Energy Sciences, Physical Biosciences
- NIH [GM060396, ES010337]
- National Science Foundation (NSF) [MCB-1616236]
- NSF [IOS-1553506]
- Human Frontier Science Program Long-Term Fellowship
- Alexander von Humboldt Feodor Lynen Fellowship
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1553506] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1616236] Funding Source: National Science Foundation
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Plants experience hyperosmotic stress when faced with saline soils and possibly with drought stress, but it is currently unclear how plant roots perceive this stress in an environment of dynamic water availabilities. Hyperosmotic stress induces a rapid rise in intracellular Ca2+ concentrations ([Ca2+](i)) in plants, and this Ca2+ response may reflect the activities of osmo-sensory components. Here, we find in the reference plant Arabidopsis thaliana that the rapid hyperosmotic-induced Ca2+ response exhibited enhanced response magnitudes after preexposure to an intermediate hyperosmotic stress. We term this phenomenon osmo-sensory potentiation. The initial sensing and potentiation occurred in intact plants as well as in roots. Having established a quantitative understanding of wild-type responses, we investigated effects of pharmacological inhibitors and candidate channel/transporter mutants. Quintuple mechano-sensitive channels of small conductance-like (MSL) plasma membrane-targeted channel mutants as well as double mid1-complementing activity (MCA) channel mutants did not affect the response. Interestingly, however, double mutations in the plastid K+ exchange antiporter (KEA) transporters kea1kea2 and a single mutation that does not visibly affect chloroplast structure, kea3, impaired the rapid hyperosmotic-induced Ca2+ responses. These mutations did not significantly affect sensory potentiation of the response. These findings suggest that plastids may play an important role in early steps mediating the response to hyperosmotic stimuli. Together, these findings demonstrate that the plant osmo-sensory components necessary to generate rapid osmotic-induced Ca2+ responses remain responsive under varying osmolarities, endowing plants with the ability to perceive the dynamic intensities of water limitation imposed by osmotic stress.
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